Brain-type Creatine Kinase Isoenzyme in Young Dogs
نویسندگان
چکیده
منابع مشابه
Muscle creatine kinase isoenzyme expression in adult human brain.
Previous studies have suggested that MM creatine kinase is a muscle-specific protein and is not present in adult brain tissue. We have isolated a protein from human brain with an apparent molecular weight of 43,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis which is identical to the muscle M creatine kinase isoenzyme subunit at all 30 sequenced amino acid residue...
متن کاملMeasurement of brain-specific creatine kinase isoenzyme activity in serum.
We have modified the columti-chromatographic technique of Mercer (1) to produce a sensitive and rapid separation of serum CK2 and CK3, even at normal total serum CK activity (3).12 However, CKj was not separated from CK2. Here, we report a modification of our method such that all three CK isoenzymes are separated by stepwise elution from a mini-column of diethylaminoethyl-cellulose. CK1 has onl...
متن کاملThe detection of creatine kinase isoenzyme CK1 in serum.
The brain isoenzyme of creatine kinase (CK) (EC 2.7.3.2) has been identified in the serum of 21 of 950 patients tested. Samples were received over a period of about 20 months. Analysis was performed by electrophoresis on agarose gel with isoenzyme bands visualized by fluorescence after enzyme coupled reactions producing NADH. Specialized substrate preparations were used to rule out the possibil...
متن کاملCreatine kinase isoenzyme--antibody reactions in immuno-inhibition and immunonephelometry.
I have raised, in rabbits, antibodies against MM and BB isoenzymes of creatine kinase. The antibodies produced were homogeneous by Ouchterlony double immunodiffusion and did not cross react with their opposite antigens. Both antisera, however, appeared to be mixtures of antibodies, displaying different equivalences for activation, inactivation, and, possibly, precipitation. Inactivation studies...
متن کاملCreatine kinase isoenzyme MB in myocardial infarction: methods compared.
Several methods are available for specifically identifying and assessing the activity of MB isoenzyme of creatine kinase in serum. We compared two electrophoretic techniques with three column-chromatographic ones for efficacy in the diagnosis of myocardial infarction. No technique was considered absolute, but we found surprisingly large errors of diagnosis in the column-chromatographic techniqu...
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ژورنال
عنوان ژورنال: Journal of the Japan Veterinary Medical Association
سال: 1996
ISSN: 0446-6454,2186-0211
DOI: 10.12935/jvma1951.49.180